Ke Ma1,
Mindong Du2,
Mingde Liao1 
,
Shihai Chen1,
Guoqian Yin1,
Qingfeng Liu1,
Qiang Wei1,
Gang Qin2
For correspondence:- Mingde Liao Email: make133494@163.com Tel:+86 0771 5356512
Received: 15 August 2014 Revised: 1 December 2014 Published: 30 January 2015
Citation: Ma K, Du M, Liao M, Chen S, Yin G, Liu Q, et al. Evaluation of wound healing effect of Punica granatum L Peel extract on deep second-degree burns in rats. Trop J Pharm Res 2015; 14(1):73-78 doi: 10.4314/tjpr.v14i1.11
© 2015 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose:To investigate the healing effect of Punica granatum L. peel (Pomegranate husk) extract (PHE) on deep second-degree burns in rats.
Methods:Male Wistar rats, weighing 220 – 240 g, were subjected to deep second-degree skin burns by electrical scald instrument. The animals were divided into three groups as follows: (1) deep second-degree burn model (control) group, (2) burns model treated with 1 % silver sulfadiazine (SSD) group, and (3) burns model treated with PHE group. On days 3, 7, 14 and 21, animal weight, wound area as well as histopathological features of skin were evaluated for all the groups. Minimum inhibitory concentration (MIC) of PHE on Staphyloccocus aureus, Pseudomonas aeruginosa and Escherichia coli were also assessed separately.
Results:On day 21, the mean wound area of PHE treatment group (0.21 ± 0.07 cm2) and SSD treatment group (1.15 ± 0.1 cm2) were significantly smaller than that of control rats (2.42 ± 0.2 cm2, p < 0.01) respectively. Histological results indicate that inflammatory cells disappeared substantially and were replaced by new granulation tissue in PHE treatment or SSD treatment group, while that of the control rats still showed severe inflammatory cell infiltration. Antibacterial test revealed that the MIC of PHE on Staphyloccocus aureus, Pseudomonas aeruginosa and Escherichia coli was 6.25, 12.5 and 6.25 mg•mL-1, respectively.
Conclusion:The results reveal that PHE is an effective and promising medicinal herb for the management of deep second degree burns.
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